Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL18U |
| Synchrotron site | NFPSS |
| Beamline | BL18U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9783 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.170, 69.170, 78.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.670 - 1.550 |
| R-factor | 0.1883 |
| Rwork | 0.187 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.083 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.670 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.088 | 2.010 |
| Rmeas | 0.090 | 2.065 |
| Rpim | 0.021 | 0.465 |
| Total number of observations | 597083 | 42647 |
| Number of reflections | 31279 | 2249 |
| <I/σ(I)> | 19.6 | 1.9 |
| Completeness [%] | 97.6 | |
| Redundancy | 19.1 | 19 |
| CC(1/2) | 0.999 | 0.765 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 277 | 2.6M AMMONIUM SULPHATE, 0.1M HEPES BUFFER PH7.5, 1% PEG 400 |
