9JYN
YdiU complexed with NAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-07 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979191 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.990, 96.040, 119.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.210 - 1.700 |
| R-factor | 0.22794 |
| Rwork | 0.227 |
| R-free | 0.24854 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lna |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.664 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0431) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 57854 | 8235 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 97.0 | 99 |
| Redundancy | 6.75 | 6.69 |
| CC(1/2) | 0.996 | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 75 mM Tris (pH8.5), 1.5M Ammonium Sulfate, 25% v/v glycerol |
