9JXU
Crystal structure of cysteine synthase A from Limosilactobacillus reuteri LR1 in its apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-17 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.642, 101.292, 63.530 |
| Unit cell angles | 90.00, 107.33, 90.00 |
Refinement procedure
| Resolution | 20.400 - 2.200 |
| R-factor | 0.21452 |
| Rwork | 0.212 |
| R-free | 0.27136 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xoq |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.937 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.400 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.273 | 1.331 |
| Rmeas | 0.308 | 1.489 |
| Rpim | 0.139 | 0.657 |
| Total number of observations | 155579 | 14032 |
| Number of reflections | 32499 | 2783 |
| <I/σ(I)> | 5.4 | 1.3 |
| Completeness [%] | 98.9 | |
| Redundancy | 4.8 | 5 |
| CC(1/2) | 0.973 | 0.412 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 0.15 M Lithium sulfate monohydrate, 0.1 M Citric acid pH 3.5, 18% PEG6000 |
