9JX6
Crystal Structure of wild type Ab-ohrB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.886, 53.561, 87.753 |
| Unit cell angles | 90.00, 107.38, 90.00 |
Refinement procedure
| Resolution | 22.560 - 2.100 |
| R-factor | 0.2126 |
| Rwork | 0.210 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.543 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 1.990 | 2.100 |
| Rmerge | 0.098 | 1.010 |
| Rpim | 0.056 | 0.380 |
| Number of reflections | 37202 | 1847 |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 98.5 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.2 M LiCl, 18 % w/v Polyethylene glycol 3350, 0.1 M MES pH 6.5 |
