9JW7

Pilin PilA from Burkholderia pseudomallei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2022-12-17
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 43
Unit cell lengths	50.560, 50.560, 49.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.080 - 1.450
R-factor	0.2108
Rwork	0.210
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.946
Data reduction software	MOSFLM (7.4.0)
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.080	1.480
High resolution limit [Å]	1.450	1.450
Rmerge	0.067	1.141
Rmeas	0.069	1.181
Rpim	0.018	0.303
Number of reflections	22367	1084
<I/σ(I)>	19.6	2.4
Completeness [%]	99.7	98.7
Redundancy	15	14.8
CC(1/2)	0.999	0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100 mM HEPES, 25% PEG 4,000