9JW0

Structure of the N-terminal 3 domains (V1-V3) villin bound to actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-16
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	84.710, 93.762, 114.323
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 - 2.690
Rwork	0.203
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9jus
RMSD bond length	0.002
RMSD bond angle	0.491
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.750
High resolution limit [Å]	2.690	2.690
Rmerge	0.156	1.283
Rmeas	0.168	1.411
Rpim	0.963	0.576
Number of reflections	24117	1423
<I/σ(I)>	13.2	1.1
Completeness [%]	100.0	99.4
Redundancy	7.1
CC(1/2)		0.459

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M Bis-Tris propane, pH 8.5, 20% PEG 3350, 0.2 M sodium nitrate, 1 mM CaCl2