9JVD

Leishmania donovani TFIIS LW domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979230
Spacegroup name	P 1
Unit cell lengths	33.968, 53.008, 64.431
Unit cell angles	66.56, 81.49, 85.62

Refinement procedure

Resolution	47.730 - 1.900
R-factor	0.1854
Rwork	0.184
R-free	0.20780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.920
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (2.0_5936)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.968
High resolution limit [Å]	1.900	1.900
Rmerge	0.097
Rmeas	0.120
Rpim	0.069
Number of reflections	30694	2766
<I/σ(I)>	6.29	1.23
Completeness [%]	94.8	85.85
Redundancy	2.7	2.3
CC(1/2)	0.989	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		289	1.4 M sodium malonate dibasic monohydrate pH 6