9JV7

De novo designed GFP 1GFL-19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	51.860, 69.020, 119.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.730 - 1.900
R-factor	0.1674
Rwork	0.165
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.741
Data reduction software	autoPROC (1.0.5)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.870	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.079	0.291
Rmeas	0.083	0.319
Rpim	0.025	0.125
Number of reflections	34210	2217
<I/σ(I)>	20.1	5.5
Completeness [%]	98.9
Redundancy	10.9
CC(1/2)	0.999	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Bis-Tris 6.0, 30% PEG3350