9JU0

C-terminally truncated dextran dextrinase bound with acarbose

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-06
Detector	RAYONIX MX225-HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	118.259, 138.969, 264.462
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.106 - 2.500
Rwork	0.182
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.293
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.110	2.650
High resolution limit [Å]	2.500	2.500
Rmeas	0.252	1.202
Number of reflections	150716	24036
<I/σ(I)>	8.64
Completeness [%]	99.9	99.7
Redundancy	7.6	7.6
CC(1/2)	0.990	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	10% PEG 6000, 6% Ethylene glycol, 100 mM sodium acetate buffer (pH 3.5)