9JTR

Crystal Structure of chitinase from the carnivorous plant Drosera adelae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	73.222, 73.222, 192.998
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.430 - 1.730
R-factor	0.20929
Rwork	0.208
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.750
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.250	1.760
High resolution limit [Å]	1.730	1.730
Rmerge	0.052	0.787
Rmeas	0.055	0.840
Rpim	0.018	0.292
Number of reflections	63657	3470
<I/σ(I)>	26.5	2.2
Completeness [%]	100.0	100
Redundancy	9.5
CC(1/2)	1.000	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.8 M K2HPO4 0.8 M NaH2PO4 0.1 M HEPES pH7.5