9JTD

Crystal structure of PCoV-GD receptor binding domain complexed with fox ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	I 41 2 2
Unit cell lengths	193.952, 193.952, 151.024
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.070 - 3.590
R-factor	0.2306
Rwork	0.229
R-free	0.26280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6m0j
RMSD bond length	0.005
RMSD bond angle	0.720
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.720
High resolution limit [Å]	3.590	3.590
Number of reflections	16994	1632
<I/σ(I)>	9.67
Completeness [%]	99.1
Redundancy	23.6
CC(1/2)	1.000	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.2M Ammonium chloride, 20% w/v Polyethylene glycol 3350