9JTA

Crystal structure of RNF213 RING domain bound to IpaH1.4 LRR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-13
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	68.609, 68.609, 147.694
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.380 - 1.700
R-factor	0.1945
Rwork	0.193
R-free	0.21980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7v8e
RMSD bond length	0.009
RMSD bond angle	1.114
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.220	1.731
High resolution limit [Å]	1.700	1.702
Rmerge	0.063	1.531
Number of reflections	39533	1927
<I/σ(I)>	26.6
Completeness [%]	100.0
Redundancy	25.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	289	0.1 M HEPES (pH 7.8), 0.2 M Ammonium acetate, 20% (w/v) polyethylene glycol 3,350