9JS5

Crystal structure of the ASFV-derived histone-like protein pA104R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97851
Spacegroup name	P 32 2 1
Unit cell lengths	55.648, 55.648, 152.345
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.880 - 1.800
R-factor	0.2001
Rwork	0.198
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lmh
RMSD bond length	0.008
RMSD bond angle	0.943
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.880	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.075	0.626
Number of reflections	48848	3104
<I/σ(I)>	23.24
Completeness [%]	99.9
Redundancy	17.9
CC(1/2)	0.999	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	291	2.6 M ammonium sulfate, 0.1M citric acid