9JRW

Quroum Sensing Master Regulator, HapR in Vibrio mimicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER X8 PROTEUM
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-04
Detector	Bruker PHOTON III
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	44.572, 85.282, 114.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.440 - 2.150
R-factor	0.2349
Rwork	0.233
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xxo
RMSD bond length	0.003
RMSD bond angle	0.583
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.390	2.220
High resolution limit [Å]	2.150	2.150
Number of reflections	55173	3821
<I/σ(I)>	13.1
Completeness [%]	99.0
Redundancy	5.1
CC(1/2)	0.999	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	20% PEG 8000, HEPES pH 7.5, 200 mM NaCl