9JQW

The Crystal Structure of LCC6-Active from Biortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979191
Spacegroup name	P 21 21 21
Unit cell lengths	39.611, 74.690, 157.453
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.979 - 2.050
Rwork	0.150
R-free	0.20180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vve
RMSD bond length	0.007
RMSD bond angle	1.336
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.980	2.110
High resolution limit [Å]	2.050	2.050
Rmerge	0.194	0.979
Number of reflections	30250	2289
<I/σ(I)>	10.5
Completeness [%]	99.9
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M (NH4)2SO4, 0.1M Mes pH6.5, 30% w/v Polyethylene glycol Monomethyl ether 5,000