9JQS

Apo form of Oryza sativa solanesyl diphosphate synthase 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	P 21 21 21
Unit cell lengths	56.122, 90.516, 121.969
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.698 - 1.994
R-factor	0.2096
Rwork	0.207
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.801
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.16_3549: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.066
High resolution limit [Å]	1.994	1.994
Rmeas	0.110
Number of reflections	43068	4186
<I/σ(I)>	2.79
Completeness [%]	99.9
Redundancy	6.9
CC(1/2)		0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG3350, Ammonium sulfate, Bis-Tris