9JQL

The C-terminal structure of N6-methyladenosine deaminase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	103.169, 103.169, 42.629
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.760 - 2.100
R-factor	0.2065
Rwork	0.206
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.760	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.118	0.682
Rmeas	0.124
Number of reflections	13926	13964
<I/σ(I)>	18.2	3.9
Completeness [%]	99.9	100
Redundancy	10.4	10.8
CC(1/2)	0.999	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1.5 M KH2PO4 and 0.1 M HEPES (pH7.0)