9JNI

KCMF1 Zn-coordinating domains with RCKG peptide (Sulfonic Cysteine)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.282590
Spacegroup name	P 43 3 2
Unit cell lengths	97.592, 97.592, 97.592
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.430 - 1.920
R-factor	0.2005
Rwork	0.197
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.435
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18rc7_3834)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.430	1.989
High resolution limit [Å]	1.920	1.920
Number of reflections	12643	1204
<I/σ(I)>	38.1
Completeness [%]	99.7
Redundancy	75.9
CC(1/2)	1.000	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES, pH 7.0 1 M Magnesium chloride hexahydrate 20 % w/v PEG 6000 10 % v/v Ethylene glycol