9JMA

Crystal structure of glycosyltransferase AvpGT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-02
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	48.362, 95.144, 70.484
Unit cell angles	90.00, 97.16, 90.00

Refinement procedure

Resolution	42.090 - 2.200
R-factor	0.1976
Rwork	0.195
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.090	2.279
High resolution limit [Å]	2.200	2.200
Number of reflections	32146	3202
<I/σ(I)>	5.07	3.01
Completeness [%]	99.7
Redundancy	6.5
CC(1/2)	0.955	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	20% (w/v) PEG 6000, 0.1 M Hepes, 0.2 M Calcium chloride dihydrate