9JM9
NADP-dependent oxidoreductase complexed with NADP and substrate 3
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.790, 87.790, 219.611 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.080 - 3.380 |
| R-factor | 0.2051 |
| Rwork | 0.201 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.162 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.520 | 3.560 |
| High resolution limit [Å] | 3.380 | 3.380 |
| Number of reflections | 12784 | 1798 |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.3 | |
| CC(1/2) | 0.996 | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | PEG3350,sodium chloride,bis-tris hydrochloride |
