9JLY

NADP-dependent oxidoreductase complexed with NADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 41 21 2
Unit cell lengths	90.037, 90.037, 203.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.000 - 3.110
R-factor	0.1882
Rwork	0.181
R-free	0.25070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.117
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.110	3.110
Rmeas	0.193
Number of reflections	15720	2837
<I/σ(I)>	14.9
Completeness [%]	99.6
Redundancy	12.6
CC(1/2)		0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277.15	PEG3350,sodium chloride,bis-tris hydrochloride