9JKD

Crystal structure of Aspergillus fumigatus polymycovirus 1 ploymerase elongation complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	118.270, 118.270, 136.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.320 - 2.340
R-factor	0.1754
Rwork	0.173
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Cyro-EM structure of AfuPmV-1 N85
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.420
High resolution limit [Å]	2.340	5.040	2.340
Rmerge	0.065	0.028	0.593
Rmeas	0.066	0.029	0.605
Rpim	0.013	0.006	0.123
Total number of observations	1073844
Number of reflections	41675	4454	4114
<I/σ(I)>	11.7
Completeness [%]	100.0	99.9	100
Redundancy	25.8	24	23.8
CC(1/2)		1.000	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	potassium acetate, pentaerythritol propoxylate (5/4 PO/OH)