9JJU

Truncated RNF112, nucleotide-free

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-21
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	243.489, 39.766, 48.773
Unit cell angles	90.00, 100.03, 90.00

Refinement procedure

Resolution	28.380 - 2.090
R-factor	0.226
Rwork	0.226
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.914
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((1.19_4092: 000))
Refinement software	PHENIX ((1.19_4092: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.380	2.200
High resolution limit [Å]	2.090	2.090
Number of reflections	26331	3912
<I/σ(I)>	8.3	2.5
Completeness [%]	95.6	98.4
Redundancy	3.8	3.3
CC(1/2)	0.998	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 3350, Tacsimate pH 6.0,