9JJD
Structural analysis of autophagy-related protein 8a in Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.450, 58.990, 68.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.730 - 1.360 |
| R-factor | 0.1828 |
| Rwork | 0.180 |
| R-free | 0.22050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.098 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX ((1.20.1_4487: ???)) |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.730 | 1.400 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmerge | 0.085 | 0.967 |
| Rmeas | 0.085 | 1.047 |
| Rpim | 0.025 | 0.395 |
| Number of reflections | 29174 | 2111 |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris pH=6.5, 26 (W/V) PEG MME 5000 |
