9JIH
Crystal structure of BAR domain of ACAP4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.023, 189.975, 43.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.840 - 2.800 |
| R-factor | 0.2419 |
| Rwork | 0.240 |
| R-free | 0.27480 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.544 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASES |
| Refinement software | PHENIX (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.840 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.099 | 0.592 |
| Rmeas | 0.105 | 0.631 |
| Rpim | 0.035 | 0.215 |
| Total number of observations | 140851 | 19231 |
| Number of reflections | 15659 | 2241 |
| <I/σ(I)> | 14.9 | 4.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 9 | 8.6 |
| CC(1/2) | 0.999 | 0.944 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2M Lithium sulfate monohydrate 0.1M Tris ph8.5 25%w/v polyethylene glycol 3350 |
