9JFL
Molecular basis of the phosphorothioation-sensing antiphage defence system DndBCDE-DndI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.379, 135.078, 225.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.020 - 3.000 |
| R-factor | 0.22745 |
| Rwork | 0.224 |
| R-free | 0.28553 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.308 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 1.335 | |
| Rmeas | 0.169 | |
| Rpim | 0.372 | |
| Number of reflections | 31858 | 3123 |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 13.3 | 13.8 |
| CC(1/2) | 0.838 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 4% PEG6000 and 100 mM MES monohydrate, pH 6.1 |
