9JFJ
Crystal structure of the cytoplasmic domain of ZraS in ADP-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-12-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.870, 121.790, 88.430 |
| Unit cell angles | 90.00, 126.04, 90.00 |
Refinement procedure
| Resolution | 46.360 - 2.490 |
| R-factor | 0.262 |
| Rwork | 0.261 |
| R-free | 0.28190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.492 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.360 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.164 | 0.693 |
| Number of reflections | 38311 | 4257 |
| <I/σ(I)> | 5.57 | 0.72 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.96 | 4.53 |
| CC(1/2) | 0.994 | 0.727 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2M Ammonium sulfate, 0.1M Tris pH 8.5, 25% PEG 3350 |
