9JER
Arginine decarboxylase in Aspergillus oryzae, ligand-free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-05-12 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 1.00 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 134.692, 196.484, 196.783 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.770 - 1.900 |
R-factor | 0.1704 |
Rwork | 0.169 |
R-free | 0.20230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.809 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.093 | 0.620 |
Rmeas | 0.100 | 0.667 |
Number of reflections | 203179 | 32510 |
<I/σ(I)> | 13.77 | 2.69 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 7.66 | 7.27 |
CC(1/2) | 0.998 | 0.884 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M sodium acetate buffer (pH 7.0), 12% w/v PEG 3350 |