9JEB

Crystal structure of SARS-CoV-2 RBD in complex with a neutralizing antibody scFv N1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-05-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	P 62
Unit cell lengths	190.399, 190.399, 60.138
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.980 - 2.300
R-factor	0.1794
Rwork	0.178
R-free	0.20590
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.274
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.980	2.383
High resolution limit [Å]	2.300	2.300
Number of reflections	50406	5498
<I/σ(I)>	14.5
Completeness [%]	90.5
Redundancy	9.1
CC(1/2)	0.994	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.2M Magnesium sulfate heptahydrate, 20% w/v Polyethylene glycol 3350, pH 6.0