9JE3

Structure of #2-911 Fab in complex with MEDI8852 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	273
Detector technology	PIXEL
Collection date	2024-05-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	84.283, 151.111, 176.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.430 - 2.374
Rwork	0.200
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jw3 2qhr
RMSD bond length	0.008
RMSD bond angle	1.566
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (v5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.430	2.459
High resolution limit [Å]	2.374	2.374
Rmerge	0.038	0.469
Number of reflections	91583	8971
<I/σ(I)>	12.72
Completeness [%]	88.8
Redundancy	2
CC(1/2)	0.999	0.748

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Bis-Tris (pH5.5), 13% PEG3350, 1% Tacsimate, 10 mM TCEP