9JB3

Crystal structure of DH domain of human FGD6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9790
Spacegroup name	P 41
Unit cell lengths	74.197, 74.197, 40.398
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.732 - 1.500
R-factor	0.1525
Rwork	0.151
R-free	0.18980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mpx
RMSD bond length	0.005
RMSD bond angle	0.808
Data reduction software	autoPROC
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.732	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.067	0.124
Number of reflections	35425	1730
<I/σ(I)>	34.5
Completeness [%]	100.0
Redundancy	12.7
CC(1/2)	0.993	0.986

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	0.1 M HEPES pH 7.5, 25% PEG 3350