9JAJ

GMPK in complex with GDP and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 1
Unit cell lengths	33.278, 44.478, 67.445
Unit cell angles	72.09, 84.05, 75.87

Refinement procedure

Resolution	24.390 - 2.650
R-factor	0.2146
Rwork	0.212
R-free	0.26610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lvg
RMSD bond length	0.003
RMSD bond angle	0.652
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.19_4092: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.240	2.780
High resolution limit [Å]	2.650	2.650
Rmerge	0.053	0.228
Rmeas	0.066	0.287
Rpim	0.039	0.171
Total number of observations	25030	3502
Number of reflections	9883	1353
<I/σ(I)>	9.6	3
Completeness [%]	95.2
Redundancy	2.5	2.6
CC(1/2)	0.997	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	35% PEG4000, 0.1 M ammonium acetate, 0.1 M trisodium citrate, pH 5.0