9JAI
GMPK in complex with GMP and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97851 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.770, 62.920, 66.160 |
| Unit cell angles | 90.00, 107.31, 90.00 |
Refinement procedure
| Resolution | 31.580 - 2.300 |
| R-factor | 0.1978 |
| Rwork | 0.195 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lvg |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.677 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.580 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.208 | 0.637 |
| Rmeas | 0.240 | 0.738 |
| Rpim | 0.120 | 0.368 |
| Total number of observations | 59586 | 6137 |
| Number of reflections | 15194 | 1570 |
| <I/σ(I)> | 5.4 | 2.3 |
| Completeness [%] | 99.1 | |
| Redundancy | 3.9 | 3.9 |
| CC(1/2) | 0.977 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 40% PEG4000, 0.1 M ammonium acetate, 0.1 M trisodium citrate, pH 5.2 |
