9JAG
GMPK in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97923 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.632, 56.818, 60.574 |
| Unit cell angles | 90.00, 99.71, 90.00 |
Refinement procedure
| Resolution | 35.140 - 1.900 |
| R-factor | 0.1857 |
| Rwork | 0.184 |
| R-free | 0.21780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9j8l |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.615 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.140 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.542 |
| Rmeas | 0.085 | 0.599 |
| Rpim | 0.033 | 0.250 |
| Total number of observations | 96160 | 4424 |
| Number of reflections | 14447 | 780 |
| <I/σ(I)> | 15.2 | 3 |
| Completeness [%] | 96.4 | |
| Redundancy | 6.7 | 5.7 |
| CC(1/2) | 0.998 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 5% isopropanol, 2.0 M ammonium sulfate |
