9JAF
GMPK in complex with GMP and sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97851 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.795, 43.588, 67.222 |
| Unit cell angles | 72.38, 85.02, 76.42 |
Refinement procedure
| Resolution | 23.940 - 1.900 |
| R-factor | 0.1931 |
| Rwork | 0.191 |
| R-free | 0.23910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lvg |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.578 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.910 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.083 | 0.519 |
| Rmeas | 0.099 | 0.613 |
| Rpim | 0.052 | 0.323 |
| Total number of observations | 91940 | 5969 |
| Number of reflections | 26314 | 1702 |
| <I/σ(I)> | 8.7 | 2.3 |
| Completeness [%] | 96.8 | |
| Redundancy | 3.5 | 3.5 |
| CC(1/2) | 0.996 | 0.795 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 25% PEG4000, 0.2 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6. |
