9JAC
GMPK in complex with GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.362, 96.362, 45.566 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.770 - 1.470 |
| R-factor | 0.1658 |
| Rwork | 0.164 |
| R-free | 0.20060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lvg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.929 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.180 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.046 | 0.503 |
| Rmeas | 0.049 | 0.571 |
| Rpim | 0.016 | 0.259 |
| Total number of observations | 371908 | 9157 |
| Number of reflections | 40564 | 1965 |
| <I/σ(I)> | 26.7 | 3.6 |
| Completeness [%] | 98.3 | |
| Redundancy | 9.2 | 4.7 |
| CC(1/2) | 0.998 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 35% PEG4000, 0.1 M Ammonium acetate, 0.1 M Trisodium citrate, pH 6.0 |
