9JAA
P[28] rotavirus VP8*
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-03 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.680, 41.325, 98.419 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.650 - 1.280 |
| R-factor | 0.1731 |
| Rwork | 0.172 |
| R-free | 0.19460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 6.187 |
| Data reduction software | autoPROC |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.370 | 1.350 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.065 | |
| Number of reflections | 37676 | 5292 |
| <I/σ(I)> | 17 | 2.7 |
| Completeness [%] | 96.7 | |
| Redundancy | 8.7 | |
| CC(1/2) | 0.999 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1 mol/L MIB (pH5.0), 30%PEG1500 |
