9J8L
apo form of GMPK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 81.728, 81.728, 219.227 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.670 - 2.900 |
| R-factor | 0.2471 |
| Rwork | 0.244 |
| R-free | 0.29480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6uni |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.636 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.19_4092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.850 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.168 | 0.906 |
| Rmeas | 0.175 | 0.942 |
| Rpim | 0.050 | 0.254 |
| Total number of observations | 126123 | 21153 |
| Number of reflections | 10320 | 1610 |
| <I/σ(I)> | 13.2 | 3.4 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.2 | 13.1 |
| CC(1/2) | 0.995 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 25% w/v PEG 3350, 0.2 M NaCl, 0.1 M Bis-Tris, pH 6.5 |
