9J8F

Structural insights into BirA from Haemophilus influenzae, a bifunctional protein as a biotin protein ligase and a transcriptional repressor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00003
Spacegroup name	P 41
Unit cell lengths	56.472, 56.472, 200.285
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.740 - 2.650
R-factor	0.2493
Rwork	0.244
R-free	0.29180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AF-P46363-F1
RMSD bond length	0.004
RMSD bond angle	0.749
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.084	0.412
Number of reflections	18223	941
<I/σ(I)>	22.313	8.6
Completeness [%]	99.8
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	296	0.2 M sodium acetate, 0.1 M MES, and 20% (w/v) PEG 3350