9J8E

Structural insights into BirA from Haemophilus influenzae, a bifunctional protein as a biotin protein ligase and a transcriptional repressor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00003
Spacegroup name	P 41
Unit cell lengths	55.580, 55.580, 201.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.790 - 2.650
R-factor	0.2059
Rwork	0.199
R-free	0.27020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9j8f
RMSD bond length	0.008
RMSD bond angle	1.124
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.1_4122: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.790
High resolution limit [Å]	2.650	2.650
Rmerge	0.047	0.264
Number of reflections	17650	807
<I/σ(I)>	41.901
Completeness [%]	99.4
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.3 M sodium chloride, 0.1 M Bis-Tris, and 20% (w/v) PEG 3350