9J6I

Crystal structure of the ABA receptor PYL1 in complex with DBSA compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 65
Unit cell lengths	126.990, 126.990, 60.195
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.680 - 2.300
R-factor	0.209
Rwork	0.207
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kdj
RMSD bond length	0.009
RMSD bond angle	1.035
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14rc3_3206)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.370
High resolution limit [Å]	2.290	2.290
Rmerge	0.027	0.435
Number of reflections	25426	2464
<I/σ(I)>	13.35	1.82
Completeness [%]	100.0
Redundancy	6.3
CC(1/2)	0.999	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	PEG 8000, Potassium phosphate dibasic, Glycerol, Ethanol