9J5P

Crystal structure of B. subtilis CspR complexed with sinefungin and cellularly expressed tRNA Leu

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00003
Spacegroup name	P 65 2 2
Unit cell lengths	136.833, 136.833, 184.113
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.180 - 3.180
R-factor	0.2178
Rwork	0.216
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	AF-O31590_F1 5on2
RMSD bond length	0.003
RMSD bond angle	0.570
Data reduction software	xia2
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.780	3.353
High resolution limit [Å]	3.180	3.182
Number of reflections	14622	871
<I/σ(I)>	10.5
Completeness [%]	94.0
Redundancy	38.7
CC(1/2)	0.994	0.362

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES (pH 7.5) 1.4 M Sodium citrate tribasic dihydrate