9J5G
Autophagy-related proteins of Magnaporthe oryzae-MoAtg8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-04-20 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.597, 52.424, 36.443 |
Unit cell angles | 90.00, 114.47, 90.00 |
Refinement procedure
Resolution | 26.210 - 2.500 |
R-factor | 0.2072 |
Rwork | 0.205 |
R-free | 0.25120 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.673 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.210 | 2.589 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.087 | 0.345 |
Number of reflections | 3341 | 352 |
<I/σ(I)> | 11.77 | |
Completeness [%] | 77.0 | |
Redundancy | 4.8 | |
CC(1/2) | 0.955 | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M Sodium acetate trihydrate,20 % w/v PEG 3350,10 % v/v Ethylene glycol |