9J4O

Crystal structure of B. subtilis Leucine tRNA with UAA anticodon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-16
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	46.190, 37.200, 81.990
Unit cell angles	90.00, 99.45, 90.00

Refinement procedure

Resolution	33.796 - 2.500
R-factor	0.21861
Rwork	0.216
R-free	0.26328
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.899
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.796	2.542
High resolution limit [Å]	2.499	2.499
Rmerge	0.112	1.945
Rmeas	0.123	2.123
Rpim	0.050	0.839
Number of reflections	8962	462
<I/σ(I)>	9.7	1
Completeness [%]	91.6	99.6
Redundancy	6.1	6.3
CC(1/2)	0.997	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	297	0.072 M sodium chloride, 0.036 M sodium cacodylate trihydrate pH 7.0, 27% v/v (+/-)-2-methyl-2,4-pentanediol, 0.0108 M spermine tetrahydrochloride, 0.5% v/v ethyl acetate