9J4L

Crystal structure of GH9l Inulin fructotransferases (IFTase)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-23
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 65
Unit cell lengths	167.680, 167.680, 85.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9.620 - 2.150
Rwork	0.205
R-free	0.23300
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.863
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	RESOLVE
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.950	2.210
High resolution limit [Å]	2.150	2.150
Number of reflections	73905	9303
<I/σ(I)>	9.58
Completeness [%]	98.5
Redundancy	4.7
CC(1/2)	0.993	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M lithium sulfate monohydrate, 0.1 M Tris (pH 8.5),and 25% (w/v) polyethylene glycol 3350