9J4J

Crystal structure of GH9l Inulin fructotransferases(IFTase)incomplex with nystose(F3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-31
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54178
Spacegroup name	P 65
Unit cell lengths	167.642, 167.642, 85.297
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.441 - 2.803
Rwork	0.158
R-free	0.20790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.772
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	RESOLVE
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Rmerge	0.155	0.495
Number of reflections	33668	1682
<I/σ(I)>	11.22
Completeness [%]	100.0
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M lithium sulfate monohydrate 0.1 M Tris (pH 8.5) 25% (w/v) polyethylene glycol 3350