9J4I
Crystal structure of GH9l Inulin fructotransferases (IFTase) in compex with fruetosyl nystose (GF4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-31 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 65 |
| Unit cell lengths | 167.713, 167.713, 85.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.120 - 1.960 |
| R-factor | 0.15996 |
| Rwork | 0.159 |
| R-free | 0.18469 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.609 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | RESOLVE |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.990 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.076 | 0.452 |
| Number of reflections | 94026 | 4791 |
| <I/σ(I)> | 18.2 | 3.277 |
| Completeness [%] | 96.2 | |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M lithium sulfate monohydrate 0.1 M Tris (pH 8.5) 25% (w/v) polyethylene glycol 3350 |
