9J4I
Crystal structure of GH9l Inulin fructotransferases (IFTase) in compex with fruetosyl nystose (GF4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-31 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 65 |
Unit cell lengths | 167.713, 167.713, 85.160 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.120 - 1.960 |
R-factor | 0.15996 |
Rwork | 0.159 |
R-free | 0.18469 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.609 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | RESOLVE |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.990 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.076 | 0.452 |
Number of reflections | 94026 | 4791 |
<I/σ(I)> | 18.2 | 3.277 |
Completeness [%] | 96.2 | |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M lithium sulfate monohydrate 0.1 M Tris (pH 8.5) 25% (w/v) polyethylene glycol 3350 |