9J4H
Crystal structure of SHMT apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 276.920, 68.090, 95.340 |
| Unit cell angles | 90.00, 110.61, 90.00 |
Refinement procedure
| Resolution | 47.670 - 2.040 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.20980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7v3d |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.583 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.670 | 2.160 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 105322 | 16500 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 11.1 | |
| CC(1/2) | 0.993 | 0.567 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 17.5% PEG3350, 1 mM nitrophenol, 0.225 M sodium thiocyanate |
