9J24
Structural basis of the bifunctionality of M. salinexigens ZYF650T glucosylglycerol phosphorylase in glucosylglycerol catabolism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 172.727, 115.065, 182.134 |
| Unit cell angles | 90.00, 100.56, 90.00 |
Refinement procedure
| Resolution | 48.390 - 2.500 |
| R-factor | 0.1761 |
| Rwork | 0.174 |
| R-free | 0.21150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7xdq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.262 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHASER (2.7.0) |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.800 | 2.350 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.128 | |
| Rmeas | 0.152 | |
| Rpim | 0.081 | 0.822 |
| Number of reflections | 157587 | 11662 |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 8% PEG 6000, 1.6 M Sodium chloride, 20% Glycerol |
