9J23
Structure of a triple-helix region of human Collagen type II from Trautec
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.987, 20.042, 44.985 |
| Unit cell angles | 90.00, 99.98, 90.00 |
Refinement procedure
| Resolution | 44.300 - 1.750 |
| Rwork | 0.241 |
| R-free | 0.28790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k6f |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.489 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 6457 | 346 |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 99.7 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.908 | 0.812 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Index-G12:0.2M MgCl2, 0.1M HEPES pH7.5, 25% PEG3,350 |
